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. 2021 Jan 7;70(3):710–719. doi: 10.2337/db20-0181

Figure 3.

Figure 3

miR-378a induced by inflammatory cytokine TNF-α disturbs metabolic homeostasis of mitochondria and ER. miR-378a-KO mice and their WT littermates (n = 5–7/group) were fasted for 8 h followed by treatment with TNF-α (8 μg/200 g body weight) for 5 h. Liver tissues were collected for protein and RNA extractions. A: miR-378a detected in the livers of mice treated with mock (saline) or TNF-α by a TaqMan miRNA probe specific for miR-378a and normalized to small nucleolar RNA202. B: mRNAs of the indicated genes in the TNF-α–treated WT and miR-378a-KO mice. C: Mitochondrial and ER stress markers HSP60, Grp78, JNK-p, and eIF2α-p as well as total eIF2α and β-actin in the livers of TNF-α–treated WT and KO mice were detected by immunoblotting analysis. Data are mean ± SD. The two-tailed Student t test was used for statistical analyses of two-group comparisons. *P < 0.05, **P < 0.01 vs. controls.