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. 2020 Dec 16;70(3):733–744. doi: 10.2337/db20-0856

Figure 2.

Figure 2

SHP inhibits AHR transactivation of miR-802 gene expression. A and B: Occupancy of SHP at miR-802 in the SHP binding region (−58/−143) (A) or at sequence outside of the region (−4408/−4522) (B) and at Cyp7a1 (C). D: Potential binding sites for transcription factors in the SHP binding region (chr16:93369637–93370499) in miR-802 were analyzed by the JASPAR program. The motif scores for potential binding motifs for the indicated factors are plotted. E: Hepa1c1c7 cells were transfected with siRNA for KLF4 or AHR for 48 h, and protein levels of AHR and KLF4 were determined by immunoblotting (left) and miR-802 levels by RT-qPCR (right, n = 6). F: Hepa1c1c7 cells were transfected with the indicated plasmids, and 2 days later, luciferase and β-galactosidase activities were measured. Luciferase activity normalized to β-galactosidase activity is plotted (n = 3). A–C, E, and F: The mean and SD are plotted. Statistical significance was determined by Student t test (A–C) or one-way ANOVA (E). **P < 0.01. Ab, antibody; Ctl, control; Luc, luciferase; mut, mutated; Rel, relative; si, small interfering; WT, wild type.