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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Genesis. 2020 Apr 28;58(8):e23368. doi: 10.1002/dvg.23368

Figure 1. Creation of a mouse line expressing an Oct4ΔPE-53BP1-mCherry transgene specifically in male and female embryonic germ cells.

Figure 1.

(A) Schematic diagram of transgene construct with the Oct4DPE embryonic germ cell specific promoter (~20 kb) and the 53BP1-mCherry DNA double strand break sensing reporter (~2.2 kb). (B) Whole mount immunofluorescence images of E13.5 transgenic male and female genital ridges with panels on the left showing endogenous mCherry reporter localization, middle panels showing antibody labeling of the cytoplasmic germ cell marker MVH (DDX4), and panels on the right showing the corresponding merged image. Scale bar= 50 μm