Fig. 4.

Inhibition of CD36 attenuates Cer-induced NF-κB activation and prevents subsequent activation of TXNIP/NLRP3 inflammasomes. The J774A.1 cells and THP-1 macrophages were pretreated with SSO (200 μmol/L) or NF-κB inhibitor SN50 (36 μmol/L) 1 h, and then incubated with C2-Cer (30 μmol/L for 5 h). b Effect of SSO on the activation of phospho-p65. c Effect of SSO and NF-κB inhibitor SN50 on Cer-induced p-P65 DNA-binding activity. Effect of SSO and NF-κB inhibitor SN50 on Cer-induced CD36 expression, TXNIP expression and NLRP3 inflammasomes: f CD36; g TXNIP; h NLRP3; i Caspase-1; j IL-1β; k IL-18. **P < 0.01, ***P < 0.001 vs. control group; ^##P < 0.01, ^###P < 0.001 vs. C2-Cer treatment group