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. 2021 Feb 21;296:198350. doi: 10.1016/j.virusres.2021.198350

Fig. 5.

Fig. 5

The PERK arm of UPR was not regulated by orf8L and orf8S. (A) Levels of phosphorylated eIF2α (top panel) and eIF2α (middle panel) were examined by western blots treated with orf8L, orf8S, orf8b, vector control and TG. GAPDH was used as loading control. (B-C) mRNA levels of ATF4 (B) and CHOP (C) were examined by qRT-PCR in HEK-293 T cells after treatment with orf8L, orf8S, orf8b, vector control and TG. Orf8L and orf8S referred to SARS-CoV-2, orf8b referred to SARS-CoV. Orf8L and orf8S referred to SARS-CoV-2, orf8b referred to SARS-CoV.* p < 0.05; ** p < 0.01; *** p < 0.001. p values were determined with two-tailed student’s t test. Error bars represent standard deviation S.D.