Fig. 6.

orf8L and orf8S are IFNß antagonist. (A) HEK-293 T cells were co-transfected with firefly luciferase plasmids harboring the corresponding promoter of IFNß, pRL-null (Renilla plasmid) and either of orf8L, orf8S, orf8b or vector control. Cells were also transfected with Poly (I:C) to activate the IFN. The ratio of the reporter (Firefly) to control (Renilla) in relative luminescence units were plotted. (B-D) mRNA levels of IFNß (B) ISG15 (C) and ISG56 (D) were examined by qRT-PCR in HEK-293 T cells after treatment with orf8L, orf8S, orf8b and vector control. Cells were also transfected with Poly (I:C) to activate the IFN expression pathway. * p < 0.05; ** p < 0.01; *** p < 0.001. p values were determined with two-tailed student’s t test. Error bars represent standard.