hrs mutant cells accumulate Wg, Arrow, and DFz2 puncta. (A–H) Wing discs with hrs mutant clones marked by the absence of GFP (green in merge). (A, B) hrs mutant cells contain enlarged late endosomes, as revealed by TR-dextran (red in merge) labeling (5-min pulse, 60-min chase). (C–D′) hrs mutant clone with enlarged Wg puncta (red in merge). (C′, D′) High-magnification image of disc in (C, D). (E–F′) hrs clone with enlarged Arrow puncta (red in merge). (E′, F′) High-magnification image of disc in panels (E, F). (G–H′) hrs clone with enlarged DFz2 puncta (red in merge). (G′, H′) High-magnification image of disc in panels (G, H).