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. Author manuscript; available in PMC: 2021 Feb 21.
Published in final edited form as: Dev Biol. 2006 Mar 10;293(1):268–283. doi: 10.1016/j.ydbio.2006.02.006

Fig. 8.

Fig. 8.

Overexpression of Rab5DN and ShiDN in the wing causes cell death. (A) C5-Gal4/UAS-GFP disc. C5 is expressed throughout the wing pouch except at the D/V boundary. (B, C) High-magnification image of C5-Gal5/UAS-DRab5DN disc stained for DrIce (red) and DNA (white). Pyknotic nuclei in the C5 domain correspond to DrIce positive cells, indicating that many DRab5DN expressing cells undergo apoptosis. (D–G) C5-Gal4/UAS-lacZ discs stained for (D) Dll, (E) Sens, (F) En, and (G) DrIce. (H–K) C5-Gal4/UAS-DRab5DN discs and (L–O) C5-Gal4/UAS-ShiDN discs stained with the same antibodies. The loss of Dll, Sens, and En expression in endocytosis-defective cells is likely due to cell death.