Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 14;49(3):1455–1469. doi: 10.1093/nar/gkaa1288

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — Time-resolved HDX-MS analysis of DNA-induced conformational changes in RPA32 and RPA14. (A) Crystal structure of the Tri-C core of human RPA composed of DBD-C, RPA32 and RPA14. The peptides identified in the MS analysis corresponding to RPA32 and RPA14 are denoted in red. DNA is depicted as black sticks. (B–Q) Deuterium exchange profiles of individual peptides from RPA32 and RPA14 are shown. Error bars reflect standard deviations, calculated as described in ‘Material and Methods’. */**/*** are P-values calculated using a two-tailed unpaired t-test between the respective time points for the RPA and RPA+DNA samples. Each star denotes t-test values that differ by an order of magnitude. P-values are denoted in Supplementary Table S2. No significance indicated as ‘ns’.