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. 2021 Jan 14;49(3):1597–1608. doi: 10.1093/nar/gkaa1278

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Effects of temperature on KmAgo activity. (A) Effects of temperature on KmAgo activity mediated by 5′P-gDNA. (B) Effects of temperature on KmAgo activity mediated by 5′P-gRNA. (C) Effects of temperature on KmAgo activity mediated by 5′OH-gDNA. (D) Cleavage kinetics of ssDNA and ssRNA targets using DNA-guided and RNA-guided KmAgo. The assay in (A), (B), (C) was performed in reaction buffer containing Mn²⁺ ions for 15 min at indicated temperatures. The assay in (D) was performed for indicated times (1, 5, 15, 30, 60, 120 min) at 37°C. Results from three independent experiments were quantified. Error bars represent SD of three independent experiments.