Figure 4.

Effects of the 5′-end nucleotide identity on KmAgo activity. (A) Effects of the 5′-end nucleotide of 5′P-gDNA on DNA cleavage activity. (B) Effects of the 5′-end nucleotide of 5′P-gDNA on RNA cleavage activity. Quantification of the cleavage efficiencies is shown on the right. All experiments were performed at the 4:2:1 KmAgo:guide:target molar ratio in reaction buffer containing Mn²⁺ ions at 37°C. Means and standard deviations from three independent measurements are shown.