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. 2021 Jan 14;49(3):1597–1608. doi: 10.1093/nar/gkaa1278

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Effects of mismatches in the guide-target duplex on the slicing activity of KmAgo. (A) Effects of single- and dinucleotide mismatches in the 5′P-gDNA:tDNA duplex on the slicing activity of KmAgo. (B) Effects of single- and dinucleotide mismatches in the 5′P-gDNA–tRNA duplex on the slicing activity of KmAgo. (C) Effects of single-nucleotide mismatches in the 5′P-gRNA:tRNA duplex on the slicing activity of KmAgo. (D) Effects of single-nucleotide mismatches in the 5′P-gRNA:tDNA duplex on the slicing activity of KmAgo. All experiments were performed at the 4:2:1 KmAgo:guide:target molar ratio in reaction buffer containing Mn²⁺ ions at 37°C. Error bars represent SD of three independent experiments.