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Quantification of neochromosome copy number. (A) neochromosome copy number as determined by whole genome sequencing. (B) neochromosome copy number estimation based on mRuby2 fluorescence. CEN.PK113-7D with no copies of mRuby2 was used as negative control and IMX2224 with a single copy of mRuby2 integrated in the genome as positive control. (C) Schematic overview of the potential segregation of circular neochromosome upon cell division.
