Figure 6.
MRE11 is essential for DSB response and repair at an expression site. (A) Clonogenic assay reveals survivors following a DSB in the VSGup strain in the parental and VSGUPmre11 cell lines. Details as in Figure 1 (B) Upper panel: The number of cells in G2/M phase cells was counted by DAPI staining at several points following induction of an I-SceI break in. G2 cells contain one nucleus and two kinetoplasts. Lower panel: Immunofluorescence assay to monitoring γH2A foci. The number of positive nuclei were counted in uninduced cells and 12 hours post DSB. Error bars, SD, n ≥ 600 for each time point in the VSGup cell line n ≥ 600 for the mre11 null. 1HR n = 3, and with 1HRrad50; n = 3.