Table 1.
Overview of trypanosome RNA targets used in this work
| Target | Localization | Special feature | Specificity controls (this work) | Abundance (mRNAs/cell), PCF | |
|---|---|---|---|---|---|
| (25) | this work | ||||
| all mRNAs | Cytoplasm (nucleus) | Poly(A) probe | Oligo(dT) competition | 61113 | N.D. |
| RPL7A | Cytoplasm (nucleus) | Underrepresented in stress granules (6) | Antisense probe | 274 | 268 |
| α-TUBULIN | Cytoplasm (nucleus) | Abundant house keeping | 810 | N.D. | |
| HEL67/DBP1 | Cytoplasm (nucleus) | Average abundance | 21 | 33 | |
| EP1 | Cytoplasm (nucleus) | Abundant in PCF | Life cycle stage specific | 572 | 582 |
| VSG (MITat1.2) | Cytoplasm (nucleus) | Abundant in BSF | Life cycle stage specific | N.D. | N.D. |
| GB4 | Cytoplasm (nucleus) | Very large mRNA, low abundant | 3 | 9.8*1 | |
| FUTSCH | Cytoplasm (nucleus) | Very large mRNA, low abundant | 4 | N.D | |
| A6 edited | Mitochondrion | Pan-edited | N.D. | 18*2 | |
| A6 unedited | Mitochondrion | Pan-edited | Ethidium bromide | N.D. | 660*2 |
| COXIII edited | Mitochondrion | Pan-edited | Ethidium bromide | N.D. | 64*2 |
| COXIII unedited | Mitochondrion | Pan-edited | Ethidium bromide | N.D. | 1211*2 |
| SL RNA | Nucleus | Substrate for trans-splicing | N.D. | 1100 | |
*1Includes large fraction of transcription and decay intermediates.
*2Includes an unknown number of editing intermediates.