Figure 4.
LCCR4 is involved in stress-triggered tRNA shortening. (A) Cells induced for LCCR4 RNAi or kept uninduced were grown exponentially or under nutritional stress and tRNA shortening analyzed by northern blot detecting tRNAVal. tRNAs containing a complete CCA-tail are marked with a full arrowhead while incomplete and totally shortened species are marked with an asterisk or open arrowheads, respectively. (B) As in (A) but cells were allowed to recover in complete media for the indicated times after nutritional stress. (C) Cells induced for LCCR4-HA overexpression or left uninduced and growing exponentially or under nutritional stress were allowed to recover in normal media for the indicated times. The upper panel shows a northern blot where tRNAVal was investigated while the lower panel corresponds to the same gel stained with ethidium bromide (EtBr). The region corresponding to the bulk cellular tRNAs is indicated. The percentage of full length tRNAVal present is indicated (%FL).