Table 1.

Kinetic parameters extracted from global fits to the c-myc Pu22 and trapped mutant TH datasets collected at 295 nm according to Scheme 1C with k_ex = 0 min⁻¹

GR isomer	E F (kJ mol−1)	k F (min−1)	E U (kJ mol−1)	k U (min−1)	T m (°C)
55	−36.0 ± 0.7	(387 ± 6) × 10−3	121 ± 1	(162 ± 3) × 10−3	43.23 ± 0.04
35	−47.9 ± 0.8	(572 ± 6) × 10−3	140.4 ± 0.8	(72.1 ± 0.9) × 10−3	47.48 ± 0.03
53	−54.6 ± 0.6	(917 ± 8) × 10−3	144.5 ± 0.4	(23.2 ± 0.2) × 10−3	53.27 ± 0.02
33	−54.4 ± 0.5	(1220 ± 9) × 10−3	164.3 ± 0.3	(7.2 ± 0.1) × 10−3	57.69 ± 0.03

Rate constants are reported at T₀ = 37 °C. Equilibrium T_m values were extracted from two-state fits of slow scanning (0.2 °C min⁻¹) thermal melts monitored at 295 nm (Supplementary Figure S10). Errors were calculated using a Monte-Carlo approach (87).