Figure 4.

Genome-wide fixed nucleotide substitution (FNS) frequencies at non-G4 non-B DNA loci and their flanking sequences. The positions of FNSs within motifs were scaled based on motif size (see Materials and Methods for details). Inverted, direct, and mirror repeats are split into spacers and repeat arms, and A-phased repeats are split into A-tracts and spacers. Flanking regions are the 2 kb up- and downstream from the loci. For clarity of visualization, only the first 100 bp are shown (the full 2 kb are shown in Supplementary Figure S8) and the Y-axes are displayed on a log scale. Gray areas indicate significantly different FNS frequency in non-B DNA versus controls (IWTomics adjusted P-value curve < 0.01).