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. 2021 Jan 15;49(3):1517–1531. doi: 10.1093/nar/gkaa1271

Figure 4.

Figure 4.

Estimated number of mtDNA building blocks of the chromosome 14 Mega-NUMT. The ostensible mtDNA copy number per NUMT was estimated by droplet digital PCR (45.00 ± 5.27; n = 20) and real-time PCR quantification (56.16 ± 4.35; n = 76) in ρ° cell DNA extracts. The results obtained for fibroblasts of an unrelated individual (control) demonstrated efficacy of ρ° cell culture. Information contained in here summarizes data on four (ddPCR) or two (qPCR) mtDNA and one nDNA quantification target sequences and four different concentrations of ethidium bromide (0, 50, 75 and 100 ng/ml) applied to ρ° cell cultures. The lower/upper hinges of the boxes indicate the first/third quartiles (Q1/Q3), horizontal lines within boxes denote the median and the whiskers extend from Q1/Q3 to the minimum/maximum values in the dataset or, in the presence of outliers, from Q1/Q3 to the upper/lower fence of the distribution (i.e. Q1 – 1.5 × IQR, Q3 + 1.5 × IQR; IQR = Q3 – Q1). Potential outliers are shown as individual black dots below/above whiskers and the arithmetic mean is indicated by a white dot with black border. -EtBr: standard cell culture conditions without addition of ethidium bromide; +EtBr: ρ° cell culture with ethidium bromide (combined data for 50, 75 and 100 ng/ml culture medium); qPCR: real-time quantitative PCR results; ddPCR: droplet digital PCR results.