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. 2020 Dec 17;16(1):168–181. doi: 10.1016/j.stemcr.2020.11.010

Figure 3.

Figure 3

Recovered hiPSC-CMs Show Altered Electrophysiology

(A) Representative single-cell AP recordings of line 1 fresh and recovered hiPSC-CMs using ASAP2.

(B) APD50 and APD90 were altered in recovered hiPSC-CMs from four out of five lines (line 1, n = 50 and 50; line 2, n = 59 and 48; line 3, n = 33 and 25; line 4, n = 14 and 21; line 5, n = 22 and 21 cells in fresh and recovered groups, respectively). Data were collected from three independent dish preparations from three independent differentiations for lines 1, 2, and 3, and two independent differentiations for lines 4 and 5.

(C) Beating rate of fresh and recovered hiPSC-CMs measured using ASAP2.

(D) Representative MEA recordings showed that FPD was shorter in recovered hiPSC-CMs.

(E) Recovered hiPSC-CMs from four out of five lines showed altered FPDc (line 1, n = 101 and 114; line 2, n = 84 and 78; line 3, n = 94 and 76; line 4, n = 93 and 92; line 5, n = 94 and 93 independent measurements in fresh and recovered groups, respectively).

Data were collected from two independent differentiations for each line. All data represented as mean ± SEM, ∗p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 compared with their respective fresh samples.