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. 2021 Feb 8;11:599771. doi: 10.3389/fimmu.2020.599771

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Copyright © 2021 Kovács, Vadászi, Bulyáki, Török, Tóth, Mátyás, Kun, Hunyadi-Gulyás, Fedor, Csincsi, Medzihradszky, Homolya, Juhász, Kékesi, Józsi, Györffy and Kardos

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colocalization of synaptic C1q with NPs in mouse brain sections. (A, B) High-resolution confocal microscopy images of triple immunostained cerebral cortical sections were segmented to identify individual C1q, NP1/2, and Syp spots. White circles indicate several triple-colocalizing spots automatically identified according to the predefined criterion. Analyses demonstrated extensive colocalization of synaptic C1q with synaptic NPs, particularly with NP1. (T-test results: (NP1) P = 2,19007E-10, (NP2) P = 4,71338E-06 on normally distributed samples.) (C, D) Close proximity of synaptic C1q with NP1 (C) and NP2 (D) is not the mere consequence of their high abundance. Medians of the minimal center-to-center distance’s cumulative frequency distributions significantly differ between the observed and randomly shuffled samples (P = 0.00019, Wilcoxon signed-rank test). Means ± SEM are shown; n = 18 3D-images recorded from sections of three mice. Scale bar = 0.5 µm. (E) The lateral view of the colocalized proteins.