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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2020 Aug 22;16(3):581–591. doi: 10.1007/s11481-020-09951-6

Fig. 2.

Fig. 2.

Morphine and gp120 cooperatively promote astrocytic activation and cytokine expression in the SDH. A. Immunoblots of GFAP, IL-1β, TNF-α, CCR5, and MOR in the spinal dorsal horn (SDH). B-F. Quantitative summaries of A. G. Immunostaining of GFAP in the SDH. H. Quantification of GFAP+ cells in the SDH of E. The samples were collected from the animals as shown in Fig. 1 (*, p<0.05; **, p<0.01; ***, p<0.001; NS, non-significant; One-Way ANOVA with Tukey post-hoc tests, n=3; scale bar, 100 μm). Control animals were administered with heat-inactivated gp120 (i.t.) and saline (i.p.).