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. 2021 Feb 10;97(2):89–102. doi: 10.2183/pjab.97.005

Figure 5.

Figure 5.

Glial activation in the thalamus and hippocampus of JF1/B6F1 Ngly1−/− mice. Immunohistochemistry for GFAP (A) and IbaI (B) in the thalamus and hippocampus of JF1/B6F1 Ngly1−/− mice and their littermate WT mice at 5 weeks of age. Scale bar, 0.5 mm. Nuclei were stained with DAPI. (C and D) Quantitative analyses showing the area occupied by GFAP-positive (C) or IbaI-positive (D) areas in the thalamus of WT and Ngly1−/− mice. Mean ± SEM (n = 4–6). *P < 0.05; **P < 0.01. (E) Immunohistochemistry of the thalamus from JF1/B6F1 Ngly1−/− and their littermate WT mice at 5 weeks of age, stained with anti-NeuN, a mature neuron marker (green). Nuclei were stained with DAPI. Scale bar, 100 µm.