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. 2021 Feb 15;6(9):2767–2782. doi: 10.1016/j.bioactmat.2021.02.004

Fig. 2.

Fig. 2

Characterization of ECM before and after decellularization. (a) HE staining. (b) Masson's trichrome staining. (c) Scott's alcian blue staining. (d) Collagen content analysis (n = 4). (e) GAG content analysis (n = 4). (f) DNA content analysis (n = 3). Scale bar: 50 μm **P < 0.01 represents statistically significant difference between before and after decellularization in each group (ANOVA followed by Tukey's post hoc test). Before: before decellularization. After: after decellularization. Low: low stiffness group; Medium: medium stiffness group; High: high stiffness group.