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. 2021 Feb 15;6(9):2767–2782. doi: 10.1016/j.bioactmat.2021.02.004

Fig. 4.

Fig. 4

Recellularization performance and cell viability of MDA-MB-231 within DECM scaffolds with different stiffness. (a) CSE of MDA-MB-231 cells in three scaffolds (n = 3). (b) Cell number was detected by quantitative DNA assay for recellularization cultured in the DECM scaffolds with different stiffness at day 10 (n = 3). (c) Cell viability detected by MTS assay for recellularization cultured in the TCPS and DECM scaffolds with different stiffness (n = 3). All the values were normalized to the cell viability on the TCPS at day 1. (d) Cell viability observed by Live/dead staining for recellularization cultured in the TCPS and DECM scaffolds with different stiffness at days 1, 4, 7 and 10. The red square frames identify dead cells. Scale bar: 100 μm. (e) Cell infiltration in DECM with different stiffness was detected by HE staining at days 1, 4, 7 and 10. Scar bar: 50 μm. (f) Cell infiltration in DECM with different stiffness was observed by laser scanning confocal microscope at day 7. Scale bar: 200 μm. Low: low stiffness group; Medium: medium stiffness group; High: high stiffness group.