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. 2021 Feb 15;6(9):2767–2782. doi: 10.1016/j.bioactmat.2021.02.004

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© 2021 [The Author/The Authors]

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 7 — Effect of ABCB1 on the drug resistance of MDA-MB-231 cells cultured in DECM scaffolds with different stiffness. (a) qPCR analyses for mRNA expression of YAP, FAK, ABCB1, ABCC3, ABCG2 and Bcl-2 which were normalized to GAPDH (n = 3). #P < 0.05 represents statistically significant difference compared with the TCPS group; *P < 0.05 represents statistically significant difference compared with the Low group. & P < 0.05 represents statistically significant difference compared with the Medium group. (b) Western blotting analysis of drug resistance-related proteins expression of the cells (n = 3). #P < 0.05 represents statistically significant difference compared with the TCPS group. ##P < 0.01 represents extremely statistically significant difference compared with the TCPS group. *P < 0.05 represents statistically significant difference compared with the Low group. (c) Western blot detected the expression of ABCB1 protein. (d) Statistical data of the expression of ABCB1 protein (n = 3). *P < 0.05 represents statistically significant difference compared with the TCPS group without elacridar treatment. #P < 0.05 represents statistically significant difference compared with the Low group without elacridar treatment. & P < 0.05 represents statistically significant difference compared with the group without elacridar treatment in the same scaffold. (e) The curve of cell viability. (f) Statistical data of IC50 values (n = 3). ##P < 0.01 represents extremely statistically significant difference compared with the TCPS group without elacridar treatment. *P < 0.05 represents statistically significant difference compared with the Low group without elacridar treatment. & P < 0.05 represents statistically significant difference compared with the group without elacridar treatment in the same scaffold. (g) Cell apoptosis of MDA-MB-231 cultured in the DECM scaffold with high stiffness before and after treated with 2 μM elacridar. (h) Statistical data of apoptosis (n = 3). **P < 0.01 represents extremely statistically significant difference compared with the group without elacridar treatment. Statistical significance was determined by ANOVA followed by Tukey's post hoc test. Low: low stiffness group; Medium: medium stiffness group; High: high stiffness group. Ctrl: group without elacridar treatment. Ela: group treated with 2 μM elacridar.