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. 2021 Feb 15;6(9):2767–2782. doi: 10.1016/j.bioactmat.2021.02.004

Fig. 8.

Fig. 8

Effect of Bcl-2 on the drug resistance of MDA-MB-231 cells cultured in DECM scaffolds with different stiffness. (a) Western blot detected the expression of Bcl-2 protein, (b) Statistical data of the expression of Bcl-2 protein (n = 3). *P < 0.05 represents statistically significant difference compared with the TCPS group without ABT-737 treatment. #P < 0.05 represents statistically significant difference compared with the Low group without ABT-737 treatment. & P < 0.05 represents statistically significant difference compared with the group without ABT-737 treatment in the same scaffold. (c) The curve of cell viability. (d) Statistical data of IC50 values (n = 3). ##P < 0.01 represents extremely statistically significant difference compared to the TCPS group without ABT-737 treatment. *P < 0.05 represents statistically significant difference compared with the Low group without ABT-737 treatment. (e) Cell apoptosis of MDA-MB-231 cultured in the DECM scaffold with high stiffness before and after treated with 1 μM ABT-737. (f) Statistical data of apoptosis (n = 3). **P < 0.01 represents extremely statistically significant difference compared with the group without ABT-737 treatment. (g) The typical graph of cell cycle. (h) Statistical data of cell proliferation (n = 3). Statistical significance was determined by ANOVA followed by Tukey's post hoc test. Low: low stiffness group; Medium: medium stiffness group; High: high stiffness group. Ctrl: group without ABT-737 treatment.