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. 2021 Feb 19;41(2):BSR20202924. doi: 10.1042/BSR20202924

Figure 3. HMGB1 mediates the glucose-induced ferroptosis in mesangial cells.

Figure 3

SV40 MES 13 cells were cultured and transfected with HMGB1 siRNA or scramble siRNA (Scr-siRNA), followed by high glucose (25 mM) treatment. (A) Determination of protein levels of HMGB1 by Western blot. (B) Cell viability in each group was determined with CCK-8 assay. (C) ELISA was used to detect the production of LDH. (D) Extracellular HMGB1 protein levels were determined by Western blot. (E) Real-time PCR was performed to determine levels of ACSL4, PTGS2, NOX1, and GPX4. *P<0.05, **P<0.01, compared with control. #P<0.05, ##P<0.01, compared with HG group.