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. 2021 Feb 15;6(9):2754–2766. doi: 10.1016/j.bioactmat.2021.02.005

Fig. 2.

Fig. 2

Biocompatibility of each sample. (a) Live/dead assay of BMSCs cultured on each sample surface for 1 day. The red staining indicated dead cells and green staining indicated live cells. Scale bar represents 100 μm. (b) Quantitative analysis of live/dead assay (n = 3). (c) CCK8 assay was used to evaluate the proliferation of BMSCs cultured on the surface of each sample for 1, 3 and 7 days (n = 5). (d) Immunofluorescence images showed the adhesion of BMSCs cultured on each sample after 3 days following culture. The green staining indicated cytoskeleton and the blue staining indicated nuclei. Scale bar represents 200 μm. (e) Quantitative analysis of cell spread area (n = 9). ANOVA followed by Bonferroni's multiple comparison test was used for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001).