Fig. 6.

Results of rat air-pouch model. (a) HE staining and Masson's trichrome staining of rat air-pouch skin. Scale bar represents 1 mm. (b) Quantitative analysis of the thickness of the fibrous layer (n = 3). (c) Immunofluorescent staining of Arg-1 and iNOS in air-pouch skin. Arg-1 and iNOS were stained red and the nuclei was stained blue. Scale bar represents 200 μm. (d) Quantitative analysis of immunofluorescent staining (n = 3). (e) The expression of Arg-1 and iNOS protein was evaluated by Western blot at 7 days. (f) Quantitative analysis of Western blot (n = 3). ANOVA followed by Bonferroni's multiple comparison test was used for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001).