Fig. 7.

In vivo new bone regeneration property of different implants. (a) An illustration of the rat femur surgical procedure. (b) General photos of the implantation site on the rat femur. Scale bar represents 2 mm. (c) Micro-CT images showing bone regeneration around the implants. The red arrows mark the new regeneration bone. (d) Reconstructed 3D images of samples and new bone. Yellow color indicates samples and red color indicates new regeneration bone. (e) Quantitative analysis of new bone 8 weeks after implantation (n = 3). (f) Histological images of methylene blue/acid fuchsin staining show the new regeneration bone around each sample 8 weeks post-operatively. Black arrow indicates collagen fiber formation. Red arrow indicates new bone formation. Scale bar represents 200 μm. ANOVA followed by Bonferroni's multiple comparison test was used for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001).