Figure 1.
Colorectal tumor decellularization. (A) Schematic illustration of colorectal tumor decellularization workflow (created with Biorender.com). (B) H&E stained 4.5 µm sections of colorectal unwashed tumors and PDS after each cycle of decellularization. Black arrowheads point at cell nuclei and black arrows point toward intact collagen fibers. Scale bars indicate 200 µm. (C) DNA concentration measured from 5 × 5 × 5 mm unwashed (n = 2) and decellularized tumors following the first (n = 4) and second wash (n = 10). Values represent mean ±SEM, *p < 0.05 (one‐way ANOVA with Tukey post hoc test)