Figure 2.

Structural and compositional characterization of decellularized and HT29‐recellularized colorectal PDS. Representative images of stained 4.5 µm sections of colorectal tumor tissue prior to washing (unwashed), after two mild detergent washes (decellularized) and after 21 days of repopulation with HT29 cell line (HT29‐recellularized). Black arrowheads point at cell nuclei and black arrows point toward intact protein structures. Scale bars indicate 200 µm. (A) Histological images of acidic polysaccharides stained with Alcian Blue. (B) Histological images of collagen I and III fibers stained with Picro‐Sirius Red. (C) Immunohistochemical images of collagen IV protein. (D) Immunohistochemical images of fibronectin protein