Table 5.

Effect of Crude Extract and Solvent Fractions of Urtica simensis on Cardiac Biomarkers

Experimental Groups	Troponin I	ALT	AST
Normal control (2% tween 80%)	0.11±0.01	56.33±14.34	192.83±22.85
Negative control CP(200mg/kg i.p)	0.37±0.03a**	253.33±7.41a***	298.83±22.30a**
Positive control (EN 10mg/kg/Po)	0.12±0.01b**	62.83±6.66b***	185.00±10.09b**
CE(100mg/kg)+CP(200mg/kg)	0.22±0.04	72.00±8.74b***	222.83±19.80
CE(200mg/kg)+CP(200mg/kg)	0.17±0.04b*	66.00±10.17b***	216.00±15.37
CE(400mg/kg)+CP(200mg/kg)	0.15±0.03b**	64.67±3.04b***	188.67±12.95b**
AF(200mg/kg)+ CP(200mg/kg)	0.18±0.02b*	85.67±26.94b***	201.00±17.32b*
AF(400mg/kg)+ CP(200mg/kg)	0.15±0.03b*	84.00±12.38b***	196.50±10.54b*
HF(200mg/kg)+ CP(200mg/kg)	0.22±0.05	129.00±20.98a*b***c*	248.50±19.17
HF(400mg/kg)+ CP(200mg/kg)	0.22±0.06	127.33±8.77a*b***c*	249.17±28.83

Notes: Mean ±SEM (n= 3 for troponin I: n=6 for AST and ALT); analysis was performed using one way ANOVA followed by Tukey post hoc test; ^aCompared with normal control; ^bCompared with negative control (CP); ^cCompared with positive control; *p<0.05; **p<0.01; ***p<0.001.

Abbreviations: AF, aqueous fraction; ALT, alanine transaminase; AST, aspartate amino transferase; CE, crude extract; CP, cyclophosphamide; EN, enalapril; HF, hexane fraction; Wt., weight.