Fig. 1.

DSS colitis is associated with oxidative stress in colon tissues. (A) Schematic diagram to illustrate the experimental design. (B) Stool color in differently treated mice. The feces collected from normal control (NC) and DSS-treated mice were lysed in PBS solution. The color of the supernatants was photographed. Note the obvious color change in the DSS group. (C) The change in mice BW. The BW was daily recorded and expressed as the percentage of BW loss relative to BW on day zero. Data shown are mean ± SD (n = 3; ^##P < 0.01 vs. NC). (D) The change in colon length. The colon was taken at necropsy on day 7 and photographed (Left panel). The colon length was measured and expressed as centimeter in bar graph (mean ± SD, n = 3; ^##P < 0.01). (E, F) Detection of sulfenic acid formation in fecal and colon proteins. An equal amount of proteins from feces or colon tissues was treated with dimedone and subjected to Western analysis for sulfenic acid (-SOH) using an anti-cysteine sulfenic acid antibody. β-actin is used as a loading control. The densitometric quantification of the signals in F was shown in bar graph (mean ± SD, n = 3; ^##P < 0.01). (G) Detection of –SH groups in feces and colon tissues. Proteins extracted from feces and colon tissues were allowed to react with fluorescent maleimide and the level of –SH was detected using dot blot analysis. The densitometric values are presented as fold of control. The result of the time-course changes of –SH in feces was from a single experiment. The data of others are mean ± SD (n = 4; ^#P < 0.05). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)