Fig. 1.

Construction and characterization of the MERS-CoV replicon. A Schematic diagram of MERS-CoV replicon cDNA clone construction. The structural genes encoding E and M were replaced with a neomycin resistance gene and an Rluc gene, respectively. B Detection of MERS-CoV N protein. BHK21 cells transfected with the MERS-CoV replicon were analyzed by IFA using an anti-N protein rabbit polyclonal antibody as the primary antibody and a Cy3-conjugated goat anti-rabbit IgG as the secondary antibody. C Luciferase activity assay. BHK21 cells were transiently transfected with the MERS-CoV replicon cDNA clone. Luciferase activity was measured at the indicated time points