Fig. 2.

Development and characterization of MERS-CoV replicon-carrying cells. A Flowchart of the development of a replicon-carrying cell line. B Luciferase activity assay of BHK21-MERS-Rep cells. Individual clones of BHK21-MERS-Rep cells were plated in a 24-well plate and were lysed after 24 h for luciferase activity measurement. C MERS-CoV N protein detection in BHK21-MERS-Rep cells. BHK21-MERS-Rep cells were detected using an anti-N protein mouse monoclonal antibody and an HRP-conjugated goat anti-mouse IgG as primary and secondary antibody, respectively