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. 2020 Nov 11;11(4):1071–1094. doi: 10.1016/j.jcmgh.2020.11.005

Figure 3.

Figure 3

RNF43 influences susceptibility to DNA damage-inducing chemotherapeutics. (A) Cell viability of WT AGS, AGSD196fs, control MKN45 and AGS (shCtrl), and RNF43 knockdown MKN45 and AGS (shRNF43) cells after treatment with 5-fluorouracil (5-FU) or cisplatin. Values were normalized over untreated cells (N = 3). (B) RNF43 mRNA levels in AGS and MKN45 GC cells after treatment with 5-FU or cisplatin (Cis) for 48 hours. Cycle threshold (CT) values were normalized to GAPDH and fold change was calculated over untreated cells (N = 3). (C) Relative volume of xenograft tumors derived from control MKN45 (shCtrl) and RNF43 knockdown MKN45 (shRNF43) cells after treatment of mice with 5-FU or cisplatin. Relative volumes were calculated over tumor volume before treatment (N = 6/group). (D) Representative images of xenograft tumors derived from control MKN45 (shCtrl) and RNF43 knockdown MKN45 (shRNF43) cells after treatment of mice with 5-FU or cisplatin. Size and weight of the resected tumors are shown (n = 6/group). Error bars indicate SD. Horizontal lines represent the median values. ∗P ≤ .05, ∗∗P ≤ .01, and ∗∗∗P ≤ .001, (A and B) 2-tailed unpaired t test, (C and D) Mann–Whitney test.