Figure 6.
RNF43 is involved in H pylori–induced DDR. (A) Colony forming units (CFU)/mg of stomach of WT (n = 13) and Rnf43ΔEx8 mice (n = 12), and inflammatory score after H pylori infection. Activity and chronicity were evaluated in antrum and corpus according to the updated Sydney system. Representative images and quantification of CD3, γH2AX, cleaved-caspase 3 (c-casp3), chloroacetate esterase (CAE)-positive cells (neutrophils and mast cells), and nuclear β-catenin–positive cells per square millimeter of tissue stained by immunohistochemistry in murine gastric tissue samples are shown. Arrowheads indicate positive cells. (B) Cxcl1, Ifng, and Tnfa mRNA levels in the stomach after 3-month H pylori PMSS1 infection. Cycle threshold (CT)values were normalized to Gapdh and fold change was calculated over uninfected mice. Horizontal lines represent the median values. ∗P ≤ .05, ∗∗P ≤ .01, and ∗∗∗P ≤ .001, Mann–Whitney test. mRNA, messenger RNA.