Table 5.

Antibodies Used in This Study

Target	Clone	Assay	Dilution	Company	Reactivity
Annexin V, Pacific Blue conjugate	-----------	Flow cytometry	1:100	Thermo Fisher	H, M
(non-p-)β-catenin	D2U8Y	IHC	1:1000	Cell Signaling (Danvers, MA)	H, M
CD3	SP7	IHC	1:150	Thermo Fisher	H, M
Chk2	1C12	WB	1:1000	Cell Signaling	H
Cleaved caspase 3 (Asp175)	9661	IHC	1:300	Cell Signaling	H, M
GAPDH	14C10	WB	1:1000	Cell Signaling	H, M
HA	H6908	IP	1:100	Sigma	–
HA	H3663	WB	1:1000	Sigma	–
Ki67	D2H10	IHC	1:400	Cell Signaling	H
p-Chk2 (Thr68)	C13C1	WB	1:1000	Cell Signaling	H
RNF43	HPA008079	IHC	1:1000	Invitrogen (ATLAS)	H
RNF43	8D6	IF	1:150	–	H
K48-linked Ubiquitin	D9D5	WB	1:1000	Cell Signaling	H
K63-linked Ubiquitin	D7A11	WB	1:1000	Cell Signaling	H
γH2Ax	ab81299	WB, IHC, IF	1:10000 (WB)	Abcam	H, M
			1:5000 (IHC)
			1:300 (IF)
γH2Ax	5438	IP	1:50	Cell Signaling	H, M

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; H, human; IF, immunofluorescence; IHC, immunohistochemistry; IP, immunoprecipitation; M, mouse; WB, Western blot.