Figure 5. The gene and protein expression of antioxidative enzymes in hOBs under different exposure strategies of CSE and MBE. (A) SOD2 gene expression measured by PCR on day 14. (B) CAT gene expression by PCR on day 14. (C) GPX gene expression by PCR on day 14. (D) Dot blot images of protein levels of the antioxidative enzymes (SOD2, CAT, and GPX) in hOBs on day 14 with different exposure strategies as described. GAPDH was used as housekeeper. (E) Protein levels of SOD2 measured by Dot blot on day 14. (F) Protein levels of CAT measured by Dot blot on day 14. (G) Protein levels of CAT measured by Dot blot on day 14. Data are represented as mean ± SEM. Significance is represented as *p < 0.05, and ^#p < 0.05, ^##p <0.001, and ^###p <0.001 vs. Crl group (pooled samples of three rounds).