FIGURE 3.

Effects of SNC-121 treatment on mRNA expression of STAT1 (A), STAT2 (B), and STAT3 (C). The retina from normal, ocular hypertensive, and SNC-121 treated ocular hypertensive animals were collected at day 7, post hypertonic saline injection. Complementary DNA was synthesized from 1 µg total RNA extracted from retina. The relative changes in mRNA levels were measured by quantitative RT-PCR (qRT-PCR) using primers specific for each gene as indicated in Table 1. The qRT-PCR data was normalized using ß-actin gene expression as an internal control. The relative expression was calculated based on the comparative threshold cycle (2^−ΔΔCt) method. Bar graph represents mean data ±SEM. OH Eyes; ocular hypertensive eyes; OH Eyes + SNC-121, ocular hypertensive eyes with SNC-121 treatment. **p < 0.01; ***p < 0.001; n = 4–9. In this experiment “n” represent a biological replicate.