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. 2021 Feb 8;12:601404. doi: 10.3389/fphar.2021.601404

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Copyright © 2021 Husain, Zaidi, Singh, Guzman and Mehrotra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in STAT3 acetylation at lysine 685 (AcK685) in response to ocular hypertension and SNC-121 treatment. The retina from normal, ocular hypertensive, and SNC-121 treated ocular hypertensive animals were collected at day 7, post hypertonic saline injection. Equivalent amount of retina protein extracts (20 μg) were analyzed by Western blotting using a selective anti-Acetyl STAT3 (AcK685) or anti-β-actin antibodies. The band intensities were measured using enhanced chemiluminescence reagent and Versadoc imaging system. Bar graph represents mean data ±SEM. OH Eyes; ocular hypertensive eyes; OH Eyes + SNC-121, ocular hypertensive eyes with SNC-121 treatment. *p < 0.05. n = 6, In this experiment “n” represent a biological replicate.