FIGURE 7.

Inhibitory effects of Stattic, a selective STAT3 inhibitor, on the mRNA expression of IL-1β (A), TNF-α (B), IL-6 (C), and IFN-γ (D). The retina from normal, ocular hypertensive, and Stattic treated ocular hypertensive animals were collected at day 7, post hypertonic saline injection. Complementary DNA was synthesized from 1 µg total RNA extracted from retina. The relative changes in mRNA levels were measured by quantitative RT-PCR (qRT-PCR) using primers specific for each gene as indicated in Table 1. The qRT-PCR data was normalized using ß-actin gene expression as an internal control. The relative expression was calculated based on the comparative threshold cycle (2^−ΔΔCt) method. Bar graph represents mean data ±SEM. OH Eyes; ocular hypertensive eyes; OH Eyes + Stattic, ocular hypertensive eyes with Stattic treatment. *p < 0.05; **p < 0.01; ***p < 0.001; n = 6–9. In this experiment “n” represent a biological replicate.