TABLE 2.
List of antibodies, vendors, catalog number, and dilutions used in the current study.
| Antibody | Company | Catalog No | Application | |
|---|---|---|---|---|
| IHC | WB | |||
| Anti-acetyl STAT3 lys 685 | Cell signaling technology | 2,523 | 1:1,000 | |
| Anti-β actin | Sigma aldrich | A5316 | 1:2000 | |
| Anti-IL-6 (10E5) | Invitrogen | ARC0962 | 1:100 | |
| Anti-IL1β (H-153) | Santa cruz biotechnology | Sc-7884 | 1:20 | |
| Anti-phospho STAT3 Tyr 705 (D3A7) | Cell signaling technology | 9,154 | 1:100 | 1:1,000 |
| Anti- STAT3 (124H6) | Cell signaling technology | 9,139 | 1:2000 | |
| Anti-rabbit IgG, HRP-linked | Cell signaling technology | 7,074 | 1:5,000 | |
| Anti-mouse IgG, HRP-linked | Cell signaling technology | 7,076 | 1:5,000 | |
| Alexa fluor 488 anti mouse | Jackson immunoresearch | 111–545–044 | 1:500 | |
| Alexa fluor 488 anti rabbit | Jackson immunoresearch | 111–545–144 | 1:500 | |
| Rhodamine red anti rabbit | Jackson immunoresearch | 711–295–152 | 1:500 | |
| Rhodamine red anti mouse | Jackson immunoresearch | 715–025–151 | 1:500 | |