Figure 9.

ER stress induced by altered membrane lipid composition drives IEC necroptosis in CTα^IKO mice. (A–C) Representative TUNEL staining in the colons of control mice and CTα^IKO mice. (D) Representative Western blot of cleaved caspase-3 and (E) quantification of cleaved caspase-3 relative to β-actin in the colons of control mice and CTα^IKO mice (n = 4/group). (F) Representative Western blot of cleaved caspase-8 and (G) quantification of cleaved caspase-8 relative to tubulin in the colons of control mice and CTα^IKO mice (n = 8/group). (H) The mRNA abundance of Birc5 and Ccnd1 in the colons of control mice and CTα^IKO mice (n = 5/group). (I) Representative electron micrographs of necroptotic features in colonocytes and goblet cells of CTα^IKO mice compared with control mice. Note swollen mitochondria (M), ruptured plasma membrane (PM), and nuclei (N) without chromatin condensation. (J) Representative Western blot of RIP3 and (K) quantification of RIP3 in the colons of control mice and CTα^IKO mice (n = 4/group). (L) The mRNA abundance of Ripk1 and Ripk3 in the colons of control mice and CTα^IKO mice (n = 5/group). Values are means ± SEM. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001. MG, _____.