Table 4.
Colonic Cytokines and Chemokines in Control Mice and CTαIKO With and Without PBA
| Protein | Control, pg/mg protein | CTαIKO, pg/mg protein | Control + PBA, pg/mg protein | CTαIKO + PBA, pg/mg protein | P genotype | P treatment | P interaction |
|---|---|---|---|---|---|---|---|
| IL1β | 6.94 ± 2.93a | 48.86 ± 15.41b | 9.16 ± 4.35a | 8.32 ± 2.92a | .03 | .4 | .2 |
| MCP-1 | 2.13 ± 1.28a | 12.02 ± 2.4b | 6.57 ± 3.24a | 6.88 ± 2.3a | .04 | .88 | .05 |
| GM-CSF | 0.58 ± 0.0a | 2.53 ± 0.75a | 0.87 ± 0.29a | 5.13 ± 0.6b | <.0001 | .01 | .04 |
| IL10 | 2.86 ± 0.97a,b | 6.26 ± 1.37a | 6.74 ± 0.44a | 2.21 ± 0.41b | .57 | .93 | .002 |
| IL12 (p40) | 5.33 ± 2.28a,b | 8.31 ± 2.80a,b | 12.50 ± 0.78a | 1.38 ± 0.45b | .07 | .95 | .006 |
| TNF-α | 0.28 ± 0.13a | 1.75 ± 0.49b | 0.45 ± 0.17a | 1.86 ± 0.25b | .0004 | .63 | .91 |
| LIF | 0.71 ± 0.3a | 7.45 ± 1.65b | 0.58 ± 0.12a | 5.27 ± 0.53b | <.0001 | .23 | .28 |
| IL12 (p70) | 0.64 ± 0.48 | 4.17 ± 2.19 | 6.41 ± 3.18 | 0.1 ± 0.02 | .41 | .61 | .01 |
| IL6 | 0.85 ± 0.41 | 2.18 ± 0.70 | 2.76 ± 1.12 | 1.18 ± 0.33 | .84 | .47 | .04 |
| IL2 | 2.88 ± 0.66 | 2.11 ± 0.66 | 1.52 ± 0.68 | 4.21 ± 0.64 | .18 | .59 | .03 |
| IL3 | 0.24 ± 0.15 | 0.53 ± 0.19 | 0.73 ± 0.31 | 0.09 ± 0.01 | .34 | .88 | .02 |
| M-CSF | 0.43 ± 0.22 | 1.36 ± 0.33 | 0.94 ± 0.38 | 0.52 ± 0.02 | .35 | .54 | .03 |
| MIP-1α | 2.99 ± 0.75 | 4.07 ± 0.92 | 2.44 ± 1.01 | 5.36 ± 0.42 | .03 | .65 | .27 |
| Eotaxin | 5.07 ± 2.51 | 22.15 ± 10.87 | 0.61 ± 0.26 | 19.3 ± 3.73 | .01 | .56 | .9 |
| IFNγ | 0.41 ± 0.08 | 0.96 ± 0.19 | 0.77 ± 0.37 | 0.62 ± 0.10 | .29 | .98 | .07 |
| G-CSF | 1.23 ± 0.33 | 1.91 ± 0.43 | 1.07 ± 0.25 | 1.50 ± 0.23 | .13 | .42 | .72 |
| IL1α | 20.14 ± 1.90 | 26.57 ± 2.89 | 22.04 ± 7.77 | 25.99 ± 5.38 | .26 | .88 | .78 |
| IL5 | 0.38 ± 0.12 | 0.20 ± 0.09 | 0.08 ± 0.05 | 0.28 ± 0.10 | .92 | .32 | .08 |
| IL9 | 16.91 ± 4.77 | 8.00 ± 2.83 | 11.62 ± 9.76 | 18.17 ± 6.27 | .84 | .68 | .21 |
| MIP-2 | 28.70 ± 8.55 | 59.90 ± 11.34 | 42.53 ± 3.31 | 41.02 ± 1.86 | .15 | .98 | .11 |
| RANTES | 1.57 ± 0.87 | 2.73 ± 0.92 | 1.22 ± 0.21 | 2.26 ± 0.53 | .18 | .65 | .99 |
NOTE. Data are means ± SEM. A 2-way analysis of variance with the Tukey post-test was used. Samples that were lower than the limit of detection were assigned a value of half the limit of detection (the lowest point obtained from the standard curve).
G-CSF, granulocyte colony stimulating factor; IFN, interferon; M-CSF, macrophage colony-stimulating factor; MIP, macrophage inflammatory protein; RANTES, Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted.
a,b
Columns that do not share a letter are significantly different (α = .05).