FIGURE 4.

Catalpol reduces the apoptosis, oxidative stress and inflammation in SCI mice through promoting miR-142. The mice were subjected to SCI and then treated with antagomiR-142/antagomir-NC via intrathecal injection for 28 days starting 15 min after SCI. On the following day after SCI, CTP was treated to mice once a day for 4 weeks by gavage. (A) The BBB scores at 1, 7, 14, 21, and 28 days were shown for all groups of mice. (B) Water content in spinal cord of mice at 28 days post-Catalpol treatment. (C and D) TUNEL staining of neuronal apoptosis. (E and F) The contents of MDA and SOD were measured by commercial kits. (G–J) The inflammatory cytokines including TNF-α, IL-1β, IL-6 and IL-10, were evaluated by ELISA assays. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. SCI group; ##p < 0.01 vs. SCI + CTP group.