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. 2021 Feb 8;11:630222. doi: 10.3389/fphar.2020.630222

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Copyright © 2021 Xia, Wang, Guo, Wu, Huang and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Catalpol reduces apoptosis, oxidative stress and inflammation in SCI cell model. (A) Cell viability was assessed using CCK-8 assay. (B) Activity of caspase-3 was measured using a caspase-3 Activity Assay kit. (C and D) Apoptosis was measured using flow cytometry. (E) ROS generation was calculated using 2,7-DCF-DA staining kit (F and G) The contents of MDA and SOD were measured by commercial kits. (H–K) The inflammatory cytokines including TNF-α, IL-1β, IL-6 and IL-10, were evaluated by ELISA assays. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. Control group; ##p < 0.01 vs. OGD/G group.