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. 2020 Nov 22;11(4):1023–1044. doi: 10.1016/j.jcmgh.2020.11.011

Figure 1.

Figure 1

Propionate promotes the migration of intestinal epithelial cells. (A, B) MSIEs were wounded and treated with SCFAs. (A) Representative phase contrast images of MSIEs. Scale bar = 300 μm. (B) Quantification of average migration distance of 3 samples per treatment. (C, D) MSIEs were treated with SCFAs, stained for Ki67, and analyzed via flow cytometry. (C) Representative graphs and (D) quantification of Ki67 from 3 samples per treatment. (E, F) MSIEs were labeled with CSFE, treated with SCFAs, and analyzed via flow cytometry. (E) Representative graphs and (F) quantification of proliferation from 3 samples per treatment. (G) MSIEs were wounded and treated with propionate in increasing concentrations of FBS. Quantification of average migration with 6 samples per treatment. ∗P < .05, ∗∗∗P < .001 ∗∗∗∗P < .0001 by 1-way ANOVA with Tukey’s posttest.